CST, 4539L, Phospho-cdc2 (Tyr15) (10A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cdc2 (Tyr15) phosphate. Validated for WB,IP,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-cdc2 (Tyr15) (10A11) Rabbit Monoclonal Antibody (CST #4539) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77897 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-cdc2 (Tyr15) (10A11) Rabbit Monoclonal Antibody detects endogenous levels of cdc2 protein only when phosphorylated at tyrosine 15. Based on sequence similarity, this antibody may cross-react with CDK2 and CDK3. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Background The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). Alternate Names CDC2; CDC28A; CDK1; CDKN1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; cyclin dependent kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 protein kinase; P34CDC2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 34 Source/Isotype: Rabbit