CST, 4542S, MAP2 Antibody

Polyclonal Antibody for studying MAP2. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, MAP2 Antibody (CST #4542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MAP2 Antibody detects endogenous levels of all isoforms of MAP2 total protein. This antibody does not cross-react with tau. Non-specific labeling of mouse pancreas, colon, small intestine, and liver may be observed by immunofluorescence. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography. Background Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). Alternate Names DKFZp686I2148; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; microtubule associated protein 2; Microtubule-associated protein 2; MTAP2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75, 82, 280 SOURCE: Rabbit