CST, 45437T, NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTAG1B. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody (CST #45437) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75017 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NY-ESO-1 protein. This antibody cross-reacts with NY-ESO-2/LAGE-1S. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NY-ESO-1 protein, isoform 1. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). New York esophageal squamous cell carcinoma-1 (NY-ESO-1) is an X-linked CTA and was first identified by serological analysis of cDNA expression libraries in esophageal carcinoma (SEREX) (4,5). Like other CTAs, NY-ESO-1 expression is repressed in normal somatic tissues but becomes derepressed in a variety of human cancer types, such as multiple myeloma, non-small cell lung carcinoma, liposarcoma, and melanoma (6,7). Although the biological function of NY-ESO-1 remains enigmatic, its tumor-restricted expression pattern and high degree of immunogenicity have positioned it as a prominent target of immunotherapy-based strategies for tumor eradication (8). Alternate Names Autoimmunogenic cancer/testis antigen NY-ESO-1; cancer antigen 3; Cancer/testis antigen 1; cancer/testis antigen 1B; Cancer/testis antigen 6.1; CT6.1; CTAG; CTAG1; CTAG1A; CTAG1B; CTG1B; ESO1; L antigen family member 2; LAGE-2; LAGE2; LAGE2A; LAGE2B; New York esophageal squamous cell carcinoma 1; NY-ESO-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 (monomer), 40 (dimer) Source/Isotype: Rabbit IgG