CST, 45441T, GLTSCR1 (E6I3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GLTSCR1. Validated for WB,IP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, GLTSCR1 (E6I3A) Rabbit Monoclonal Antibody (CST #45441) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN Specificity / Sensitivity GLTSCR1 (E6I3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total GLTSCR1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro 1545 of human GLTSCR1 protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1, to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). GLTSCR1 and its paralog GLTSCR1L, along with BRD9, have been identified as unique subunits of the non-canonical BAF (ncBAF) or GBAF complex. This complex contains either GLTSCR1 or GLTSCR1L instead of an ARID subunit, while also lacking the BAF45, BAF47, and BAF57 subunits. GBAF maps to regions distinct from the PBAF and canonical BAF complexes and has also been shown to be a synthetic lethal target for BAF-driven cancers, such as synovial sarcomas and rhabdoid tumors (10-12). Alternate Names BICRA; BRD4 interacting chromatin remodeling complex associated protein; BRD4 interacting chromatin remodelling complex associated protein; BRD4-interacting chromatin-remodeling complex-associated protein; glioma tumor suppressor candidate region gene 1; Glioma tumor suppressor candidate region gene 1 protein; GLTSCR1; GSCR1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 205 Source/Isotype: Rabbit IgG