CST, 45457S, Matrin 3 (F4Q5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying matrin 3. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Matrin 3 (F4Q5F) Rabbit Monoclonal Antibody (CST #45457) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Matrin 3 (F4Q5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total matrin 3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human matrin 3 protein. Background Matrin 3 (MATR3) is a nuclear protein with two zinc finger domains, two RNA recognition motifs, and two intrinsically disordered regions. This protein is able to bind both DNA and RNA sequences and is a critical component of the nuclear matrix (1-4). MATR3 was initially implicated in human disease through its identification in a family with an autosomal dominant form of vocal cord and pharyngeal distal myopathy (5). Since then, exome sequencing has identified over a dozen point mutations in the gene that have been linked to familial amyotrophic lateral sclerosis (ALS) and combined ALS/frontotemporal dementia (6-8). Moreover, abnormal MATR3 abundance, localization, and aggregation have been reported in motor neurons of patients with sporadic ALS (9,10). Alternate Names ALS21; DKFZp686K0542; DKFZp686K23100; KIAA0723; MATR3; matrin 3; Matrin-3; MGC9105; MPD2; VCPDM; vocal cord and pharyngeal weakness with distal myopathy Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG