CST, 45531S, EMCN (E2Z7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EMCN mouse. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, EMCN (E2Z7L) Rabbit Monoclonal Antibody (CST #45531) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live) Specificity / Sensitivity EMCN (E2Z7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total EMCN protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse EMCN protein. Background Endomucin (EMCN) is a type I transmembrane O-sialoglycoprotein expressed in endothelium in venules and capillaries, but not arteries (1,2). EMCN prevents leukocyte adhesion in non-inflamed tissues, and downregulation of EMCN during inflammation facilitates migration of inflammatory cells into target tissues (3). Notch activation in endothelial cells downregulates EMCN and promotes migration of neutrophils, modulating acute inflammation in hepatic ischemia/reperfusion injury (4). EMCN deficiency in endothelial cells drives tumor lung metastasis by providing a premetastatic niche for cancer cell colonization, and is a potential prognostic biomarker for highly metastatic cancers (5). Combined analysis of EMCN/MUC15 has been suggested as a prognostic signature for gastric cancer (6). Alternate Names 0610012K22Rik; AI315669; Emcn; Endomucin; endomucin-1; Endomucin-1/2; MUC-14; Muc14; MUCEN; mucin 14, endothelial; Mucin-14 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG