CST, 45547SF, uPAR (D7X2N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying uPAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, uPAR (D7X2N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #45547) is ready to ship. Product Usage Information This product is the carrier free version of product #12713. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12713 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity uPAR (D7X2N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total uPAR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg303 of human uPAR protein. Background The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9). Alternate Names CD87; MO3; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; U-PAR; u-plasminogen activator receptor form 2; UPAR; URKR; Urokinase plasminogen activator surface receptor; urokinase-type plasminogen activator (uPA) receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-65 Source/Isotype: Rabbit IgG