CST, 45568S, ROBO2 (E4M6D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ROBO2. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, ROBO2 (E4M6D) Rabbit Monoclonal Antibody (CST #45568) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ROBO2 (E4M6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ROBO2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1172 of human ROBO2 protein. Background ROBO2 is a member of the roundabout (ROBO) receptor family (1). The activation of ROBO2 by SLIT ligand regulates various biological processes, including promoting stem cell senescence via WNT inhibition, destabilizing podocyte actin polymerization and adhesion, and activation of Ena/VASP to facilitate tumor cell extrusion from epithelia (2-5). In development, the SLIT-ROBO pathways play important roles in neuronal axon guidance and synapse function, retinal neurovascular formation, and muscle patterning (6-9). Loss of function mutations of ROBO2 have been associated with urinary tract anomalies and vesicoureteral reflux (10). Alternate Names KIAA1568; ROBO2; roundabout guidance receptor 2; Roundabout homolog 2; roundabout, axon guidance receptor, homolog 2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 180, 220 Source/Isotype: Rabbit IgG