CST, 45581T, CD11c (D3V1E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITGAX human. Validated for WB,WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD11c (D3V1E) Rabbit Monoclonal Antibody (CST #45581) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 93233 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD11c (D3V1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD11c protein. This antibody does not cross-react with CD11b. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human CD11c protein. Background CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7). Alternate Names CD11 antigen-like family member C; CD11C; complement component 3 receptor 4 subunit; integrin alpha X; Integrin alpha-X; integrin subunit alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITAX; ITGAX; Leu M5; leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; SLEB6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG