CST, 45588T, Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SEMA7A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody (CST #45588) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total semaphorin-7A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro400 of human Semaphorin-7A protein. Background Semaphorin-7A is a GPI-anchored membrane protein that plays important roles in neuronal development and immune responses via integrin-mediated signaling. It promotes axon growth by activating the mitogen-activated protein kinase (MAPK) pathway through β1 integrin receptors. When expressed on activated T cells, it promotes the production of proinflammatory cytokines by macrophages through the α1β1 integrin receptor (1). Semaphorin-7A may also play a role in resolving inflammation through its regulation of macrophage chemotaxis and chemokinesis, and by promoting macrophage differentiation and polarization toward an anti-inflammatory phenotype (2). Neuroinflammation is a detrimental component of neurodegenerative diseases, such as Parkinson's disease (PD) and multiple sclerosis (MS). The role that semaphorin-7A plays in mediating inflammatory responses in microglia in the brain suggests that it may be a therapeutic target for these diseases. In a cell model of neuroinflammation, semaphorin-7A was shown to promote cell viability and survival through its mediation of MAPK and peroxisome proliferator-activated receptor γ (PPARγ) pathways (3). Alternate Names CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; John-Milton-Hargen human blood group Ag; MGC126692; MGC126696; SEM7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; SEMA7A; SEMAK1; SEMAL; semaphorin 7A (John Milton Hagen blood group); semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; Semaphorin-7A; Semaphorin-K1; Semaphorin-L Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG