CST, 45597S, CXCL1/CXCL2 (E5M6D) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying CXCL1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CXCL1/CXCL2 (E5M6D) Rabbit Monoclonal Antibody (Biotinylated) (CST #45597) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CXCL1/CXCL2 (E5M6D) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total CXCL1 and CXCL2 proteins. This antibody is more sensitive for detection of CXCL1 protein than CXCL2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CXCL1 recombinant protein. Background CXCL1 and CXCL2 (GRO-α and GRO-β) are two of the chemokines that act as ligands for the chemokine receptor CXCR2 (1). CXCR2 is expressed by several types of leukocytes and plays an important role in the recruitment of neutrophils to sites of inflammation (2). Although neutrophil recruitment is an important component of the innate immune system's response to infection or injury, prolonged or excessive neutrophil recruitment can lead to inflammatory disease. Therefore, blocking CXCR2 has been pursued as a therapeutic approach for a variety of inflammatory diseases (3). In addition, CXCL1 and CXCL2 expression can be elevated in the tumor microenvironment, which contributes to tumor cell survival through enhanced angiogenesis and recruitment of immunosuppressive myeloid-derived suppressor cells (MDSCs) (4,5). Alternate Names C-X-C motif chemokine 1; C-X-C motif chemokine ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXCL1; fibroblast secretory protein; FSP; GRO; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); GRO1; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROA; Growth-regulated alpha protein; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; MGSA-a; NAP-3; Neutrophil-activating protein 3; SCYB1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG