CST, 45648S, Tyrosine Hydroxylase (LNC1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Tyrosine Hydroxylase. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Tyrosine Hydroxylase (LNC1) Mouse Monoclonal Antibody (CST #45648) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. This product is stable for 36 months when stored at -20C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Tyrosine Hydroxylase (LNC1) Mouse mAb recognizes endogenous levels of total tyrosine hydroxylase protein and nonspecifically labels the OPL, ONL, and ROS in fixed frozen mouse retina by immunofluorescence Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals against human tyrosine hydroxylase protein. Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). Alternate Names dystonia 14; DYT14; DYT5b; HTH-2; TH; TH2; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55-60 Source/Isotype: Mouse IgG1 kappa