CST, 4565S, Dynamin 1 (3G4B6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying DYN1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Dynamin 1 (3G4B6) Mouse Monoclonal Antibody (CST #4565) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dynamin 1 (3G4B6) Mouse Monoclonal Antibody detects endogenous levels of total dynamin 1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant carboxy-terminal fragment of human dynamin 1. Background Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3). Alternate Names DNM; DNM1; DYN1; dynamin 1; Dynamin-1; EIEE31 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Mouse IgG2a