CST, 4567S, Fumarase (D9C5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Fumarase. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Fumarase (D9C5) Rabbit Monoclonal Antibody (CST #4567) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Fumarase (D9C5) Rabbit Monoclonal Antibody recognizes endogenous levels of total fumarase protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly354 of human fumarase protein. Background Fumarate hydratase (FH), also known as fumarase, is canonically regarded as a mitochondrial enzyme of the tricarboxylic acid cycle that catalyzes fumarate to malate (1,2). Studies have demonstrated that deficiencies in FH can lead to hereditary leiomyomatosis and renal cell cancer (1,3) or, in homozygous germline mutations, severe neurological and muscular disorders (1,2). A cytosolic fumarase isoenzyme is also involved in DNA damage repair (3). Alternate Names epididymis secretory sperm binding protein; FH; FMRD; Fumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC; HsFH; LRCC; MCL; MCUL1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG