CST, 45688T, HA-Tag (C29F4) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HA-Tag (C29F4) Mouse Chimeric Monoclonal Antibody (CST #45688) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HA-Tag (C29F4) Mouse Chimeric mAb detects recombinant proteins containing the HA epitope tag. This antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. HA-Tag (C29F4) Mouse Chimeric mAb detects exogenously expressed HA-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Species Reactivity: All Species Expected Source / Purification This recombinant chimeric antibody is engineered from HA-Tag (C29F4) Rabbit mAb #3724 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, HA-Tag (C29F4) Rabbit mAb #3724 is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse chimeric IgG2a