CST, 4568T, Phospho-IGF-I Receptor beta (Tyr980) (C14A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IGF1R (Tyr980) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IGF-I Receptor beta (Tyr980) (C14A11) Rabbit Monoclonal Antibody (CST #4568) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IGF-I Receptor beta (Tyr980) (C14A11) Rabbit Monoclonal Antibody detects endogenous levels of IGF-I β receptor protein when phosphorylated at Tyr980. This antibody may cross-react with activated insulin receptors and FLT3. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr980 of human IGF-I Receptor β. Background Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). Tyr980 of IGF-IR appears to be important for receptor kinase activation. Located in the IGF-IR juxtamembrane region, phosphorylation of this tyrosine residue creates a docking site for the binding of downstream adaptor or docking proteins (9). Alternate Names CD221; IGF-I receptor; IGF1R; IGFIR; IGFR; insulin like growth factor 1 receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG