CST, 45737T, Phospho-PSD95 (Ser295) (A8F8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSD-95 (Ser295) phosphate. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PSD95 (Ser295) (A8F8Z) Rabbit Monoclonal Antibody (CST #45737) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PSD95 (Ser295) (A8F8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of PSD95 protein only when phosphorylated at Ser295. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser295 of human PSD95 protein. Background Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience-dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5). JNK1 phosphorylates PSD95 at Ser295, enhancing synaptic accumulation of PSD95 and potentiating excitatory post-synaptic currents through PSD95's increased ability to recruit AMPA receptors. In addition, synaptic depression requires dephosphorylation of Ser295 (6). Alternate Names discs large homolog 4; discs large MAGUK scaffold protein 4; discs, large homolog 4 (Drosophila); Disks large homolog 4; DLG4; FLJ97752; FLJ98574; MRD62; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD95; SAP-90; SAP90; Synapse-associated protein 90; Tax interaction protein 15 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG