CST, 4576S, Lyn (5G2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Lyn. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Lyn (5G2) Mouse Monoclonal Antibody (CST #4576) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Lyn (5G2) Mouse Monoclonal Antibody detects endogenous levels of total Lyn protein. This antibody may cross-react with other Src-family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant His-tagged human Lyn protein. Background Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). Alternate Names FLJ26625; JTK8; Lck/Yes-related novel protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Mouse IgG2b