CST, 4578S, BRIP1/FANCJ Antibody

Polyclonal Antibody for studying BRIP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, BRIP1/FANCJ Antibody (CST #4578) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BRIP1/FANCJ Antibody detects endogenous levels of human BRIP1/FANCJ protein. Species Reactivity: Human Source / Purification BRIP1/FANCJ Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BRIP1/FANCJ. Antibodies are purified by Protein A and peptide affinity chromatography. Background BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repairing DNA cross-links and double-strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of mutations in patients with early-onset breast cancer indicate that BACH1 may act as a tumor suppressor (6).BACH1 phosphorylation at Thr1133 is thought to be involved in regulating the replication checkpoint and is required for BACH1 interaction with TopBP1 (7). Alternate Names ATP-dependent RNA helicase BRIP1; BACH1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2-associated helicase 1; BRIP1; FANCJ; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit