CST, 45814S, Phospho-dCK (Ser74) (F9J4S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying dCK (Ser74) phosphate mouse. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-dCK (Ser74) (F9J4S) Rabbit Monoclonal Antibody (CST #45814) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-dCK (Ser74) (F9J4S) Rabbit Monoclonal Antibody recognizes endogenous levels of dCK protein only when phosphorylated at Ser74. This antibody detects approximately 70 kDa and 95 kDa proteins of unknown identity in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser74 of mouse dCK protein. Background Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate dA monophosphate and dG monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3). Alternate Names Dck; Deoxyadenosine kinase; Deoxycytidine kinase; Deoxyguanosine kinase; OTTMUSP00000030586 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG