CST, 45833S, SPTLC2 (F7M8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPTLC2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SPTLC2 (F7M8B) Rabbit Monoclonal Antibody (CST #45833) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SPTLC2 (F7M8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPTLC2 protein. This antibody does not cross-react with SPTLC1 or SPTLC3 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SPTLC2 protein. Background Serine palmitoyltransferase 2 (SPTLC2) is a component of the serine palmitoyltransferase (SPT) multisubunit enzyme, the principal enzyme facilitating the initial, rate-limiting step in sphingolipid biosynthesis (1,3-5). This protein complex consists of an SPTLC1/SPTLC2 or SPTLC1/SPTLC3 heterodimer as the catalytic core (1-3). Sphingolipid biosynthesis generates long-chain based compounds by condensing serine with activated acyl-CoA to form ketodihydrosphingosine (3,4). mutations have been linked to hereditary sensory and autonomic neuropathy type I (HSAN1), frontotemporal dementia (FTD), and early-onset amyotrophic lateral sclerosis (ALS) (2,5). Alternate Names hLCB2a; HSN1C; KIAA0526; LCB 2; LCB2; LCB2a; Long chain base biosynthesis protein 2; Long chain base biosynthesis protein 2a; NSAN1C; Serine palmitoyltransferase 2; serine palmitoyltransferase long chain base subunit 2; serine palmitoyltransferase, long chain base subunit 2; serine palmitoyltransferase, subunit II; Serine-palmitoyl-CoA transferase 2; SPT 2; SPT2; SPTC2; SPTLC2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 63 Source/Isotype: Rabbit IgG