CST, 45849S, SDH5 (D1S8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SDH5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SDH5 (D1S8D) Rabbit Monoclonal Antibody (CST #45849) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SDH5 (D1S8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SDH5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala155 of human SDH5 protein. Background Succinate dehydrogenase subunit 5 (SDH5, SDHAF2) is a subunit of the succinate dehydrogenase (SDH) protein complex responsible for the oxidation of succinate during the citric acid cycle. Mitochondrial SDH5 associates with the catalytic subunit of succinate dehydrogenase and is required for adding FAD cofactor to the SDH catalytic subunit (1). Mutations in the corresponding gene are associated with hereditary head and neck paragangliomas, an autosomal disorder characterized by the development of tumors with increased penetrance over time (1). Additional research studies show that SDH5 is involved in the regulation of lung cancer metastasis mediated by the glycogen synthase kinase 3β and β-catenin signaling pathways (2). Alternate Names C11orf79; FLJ20487; hSDH5; paraganglioma or familial glomus tumors 2; PGL2; SDH assembly factor 2; SDH5; SDHAF2; SDHF2; Succinate dehydrogenase assembly factor 2, mitochondrial; succinate dehydrogenase complex assembly factor 2; Succinate dehydrogenase subunit 5, mitochondrial Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG