CST, 45850T, Fibrinogen gamma chain (E1U3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FGG. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Fibrinogen gamma chain (E1U3Z) Rabbit Monoclonal Antibody (CST #45850) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 38915 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Fibrinogen gamma chain (E1U3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total fibrinogen gamma chain protein. This antibody may cross-react with an unidentified protein of 70 kDa in some cell extracts. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser190 of human fibrinogen gamma chain protein. Background Fibrinogen is a large, multimeric plasma glycoprotein that is constitutively synthesized by liver hepatocytes. As a key component of blood clots, fibrinogen plays a central role in hemostasis and thrombosis. In total, six polypeptide chains (2Aα, 2Bβ, and 2γ) are produced intracellularly in a coordinated manner, which are then covalently linked together by a network of disulfide bonds to form a fibrinogen heterohexamer (1). Research studies have shown that expression of the three fibrinogen genes is upregulated as part of the acute phase inflammatory response (2,3). Upon engagement of the clotting cascade, thrombin-mediated cleavage of the fibrinogen Aα and Bβ chains creates fibrin monomers and a platform for the polymerization of fibrin monomers into protofibrils and an insoluble web of stable fibrin fibers (4,5). Whereas thrombin is the enzyme that drives fibrin polymerization and clot formation, plasmin is the enzymatic counterpart that facilitates fibrinolysis and clot breakdown (6). In addition to its position as a central node in the normal coagulation cascade, dysregulated fibrin deposition has been observed in pathological conditions such as cancer and viral infection (7,8). The fibrinogen gamma chain is expressed as two isoforms that result from alternative mRNA splicing, the γA chain and the γ' chain. Research studies have shown that the fibrinogen gamma chain plays important roles in shaping the architecture of fibrin clots and modulates the innate immune response through interaction with its receptor, CD11b, expressed on neutrophils and monocytes (9,10). Alternate Names FGG; FIBG; Fibrinogen gamma chain; fibrinogen, gamma chain; fibrinogen, gamma polypeptide; testicular tissue protein Li 70 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG