CST, 45901S, DR3 (D5Q2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DR3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DR3 (D5Q2R) Rabbit Monoclonal Antibody (CST #45901) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DR3 (D5Q2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DR3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala80 of human DR3 protein. The antigen resides within the extracellular domain of DR3. Background The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR3/WSL-1/Apo-3/TRAMP/LARD is a TNFR family member containing the characteristic extracellular cysteine-repeats, transmembrane region, and an intracellular DD (3-7). DR3 is activated by its ligand Apo-3L/TWEAK to induce apoptosis and activation of NF-κB (8,9). Like TNF-R1, DR3 binds to the DD adaptor protein TRADD, which can then associate with other DD proteins like FADD and RIP as well as members of the TRAF family (3,4). Tissue expression of DR3 is very restricted, primarily seen on the surface of activated thymocytes and lymphocytes and plays an important role in thymocyte negative selection (3,4,10). Studies have also indicated an association with DR3 and rheumatoid arthritis (11,12). Alternate Names Apo-3; APO3; apoptosis inducing receptor; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; DDR3; death domain receptor 3 soluble form; Death receptor 3; death receptor beta; DR3; GEF720; Guanine nucleotide exchange factor 720; LARD; lymphocyte associated receptor of death; Lymphocyte-associated receptor of death; PH domain-containing family G member 5; Pleckstrin homology domain-containing family G member 5; PLEKHG5; Protein WSL; Protein WSL-1; TNF receptor superfamily member 25; TNFRSF12; TNFRSF25; TNR25; TR3; TRAMP; translocating chain-association membrane protein; Tumor necrosis factor receptor superfamily member 25; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); tumor necrosis factor receptor superfamily, member 25; WSL; WSL-1; WSL-LR; WSL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-60 Source/Isotype: Rabbit IgG