CST, 4590S, Insulin Antibody

Polyclonal Antibody for studying insulin. Validated for IHC,IF,IF,F. Highly specific and rigorously validated in-house, Insulin Antibody (CST #4590) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Insulin Antibody detects endogenous levels of total insulin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography. Background The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). Alternate Names IDDM; IDDM1; IDDM2; ILPR; ins; Insulin; insulin (A chain, B chain); Insulin A chain; Insulin B chain; IRDN; MODY10; PNDM4; preproinsulin; proinsulin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 6 SOURCE: Rabbit