CST, 4592S, Bik Antibody

Polyclonal Antibody for studying Bik. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Bik Antibody (CST #4592) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bik Antibody detects endogenous levels of Bik protein. This antibody does not cross-react with other Bcl-2 family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to residues surrounding Asp40 of human Bik protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1,2). Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins (3). Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity (4). Alternate Names Apoptosis inducer NBK; apoptosis-inducing NBK; Bcl-2-interacting killer; BCL2 interacting killer; BCL2-interacting killer (apoptosis-inducing); BIK; BIP1; BP4; natural born killer; NBK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 SOURCE: Rabbit