CST, 4593S, C-Peptide Antibody

Polyclonal Antibody for studying insulin C-peptide. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, C-Peptide Antibody (CST #4593) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity C-Peptide Antibody detects endogenous levels of total C-peptide protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surroundind Gly71 of human C-peptide protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glucose homeostasis is regulated by hormones. Elevation of blood glucose levels during feeding stimulates insulin release from pancreatic β-cells through a glucose sensing pathway (1). Proinsulin, the insulin precursor molecule, is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (2). Alternate Names IDDM; IDDM1; IDDM2; ILPR; ins; Insulin; insulin (A chain, B chain); Insulin A chain; Insulin B chain; IRDN; MODY10; PNDM4; preproinsulin; proinsulin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 4 SOURCE: Rabbit