CST, 4594S, Stat2 Antibody

Polyclonal Antibody for studying Stat2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Stat2 Antibody (CST #4594) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Stat2 Antibody detects endogenous levels of total Stat2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human Stat2. Antibodies were purified by protein A and peptide affinity chromatography. Background Stat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5). Alternate Names IMD44; interferon alpha induced transcriptional activator; ISGF-3; MGC59816; p113; PTORCH3; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 113 SOURCE: Rabbit