CST, 45956S, EPRS Antibody

Polyclonal Antibody for studying EPRS. Validated for Western Blotting. Highly specific and rigorously validated in-house, EPRS Antibody (CST #45956) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EPRS Antibody recognizes endogenous levels of total EPRS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro978 of human EPRS protein. Antibodies are purified by protein A and peptide affinity chromatography. Background EPRS (Glutamatyl-prolyl-tRNA synthetase) is a bifunctional enzyme in the aminoacyl-tRNA ligase family that attaches the cognate amino acid to the corresponding tRNA for protein translation (1,2). EPRS usually resides in the tRNA multisynthetase complex (MSC) that may facilitate the delivery of aminoacylated tRNAs to the ribosome during protein synthesis (3,4). In monocytic cells, upon interferon (IFN)-gamma activation, EPRS becomes phosphorylated and is released from the MSC to form the so-called GAIT (IFN-Gamma-Activated Inhibitor of Translation) complex with NS1-associated protein (NSAP1), ribosomal protein L13a, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The GAIT complex binds to a defined RNA element through EPRS in the 3' untranslated region (UTR) to inhibit translation of target transcripts, including vascular endothelial growth factor (VEGF)-A, ceruloplasmin, and several cytokines and their receptors. Thus, EPRS plays an important role in inflammation regulation (5-9). Alternate Names Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; EPRS; EPRS1; GLNS; GLUPRORS; GluRS; glutamate tRNA ligase; Glutamate--tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; glutamyl-prolyl-tRNA synthetase; glutamyl-prolyl-tRNA synthetase 1; Glutamyl-tRNA synthetase; HLD15; PARS; PIG32; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; Proline--tRNA ligase; proline-tRNA ligase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 180 SOURCE: Rabbit