CST, 45965S, HLTF (E9H5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMARCA3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HLTF (E9H5I) Rabbit Monoclonal Antibody (CST #45965) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HLTF (E9H5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total HLTF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala998 of human HLTF protein. Background Helicase-like transcription factor (HLTF), also known as RUSH-1 or SMARCA3, belongs to the SNF2/SWI2 family of ATP-dependent remodelers (1-3). HLTF has been shown to interact with ubiquitin enzyme complexes to promote Lys-63-linked polyubiquitination of PCNA, maintaining genomic stability during DNA damage (4,5). HLTF has double-stranded DNA translocation activity, which can restart replication forks at DNA lesions, providing tumor suppressive functions (6). The gene is frequently hypermethylated in many different tumor types (7-9). Alternate Names DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; helicase like transcription factor; Helicase-like transcription factor; HIP116; HIP116A; HLTF; HLTF1; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; RNF80; RUSH; SMARCA3; SNF2-like 3; SNF2L3; Sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; ZBU1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG