CST, 45980T, FTO (D2V1I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FTO. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FTO (D2V1I) Rabbit Monoclonal Antibody (CST #45980) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FTO (D2V1I) Rabbit Monoclonal Antibody recognizes endogenous levels of total FTO protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly87 of human FTO protein. Background FTO (fat mass and obesity-associated protein) is the first obesity gene product identified by genome-wide association studies and it is associated with the largest effect size for this class of proteins (1-4). Multiple single-nucleotide polymorphisms (SNPs) in the first intron of the gene have been associated with increased body weight and obesity. Further studies reported that risk alleles were associated with an increase in energy intake, a reduction of activity, and possibly an increased daily fat intake (4). FTO is a DNA and RNA demethylase that catalyzes the oxidative demethylation of thymidine and uracil. Among its targets is an mRNA subset involved in regulation of learning, reward behavior, motor functions, and feeding (5). Loss of the gene in mice leads to postnatal growth retardation and a significant reduction in adipose tissue. Mice deficient in the gene have lean body mass due to increased energy expenditure and systemic activation of sympathetic neurons, while overexpression of FTO in mice leads to increased food intake and results in obesity. These results demonstrate that FTO is functionally involved in energy homeostasis (6-8). Alternate Names AlkB homolog 9; ALKBH9; Alpha-ketoglutarate-dependent dioxygenase FTO; BMIQ14; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; FTO alpha-ketoglutarate dependent dioxygenase; GDFD; KIAA1752; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; Protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG