CST, 46088S, DAP12 (E8P9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Dap12. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, DAP12 (E8P9U) Rabbit Monoclonal Antibody (CST #46088) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 93638 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity DAP12 (E8P9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total DAP12 protein. Non-specific staining was observed in testis by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro110 of human DAP12 protein. Background DNAX-activating protein 12 (DAP12, TYROBP) is a signaling adaptor for several pathogen receptors expressed by cells of the innate immune system (1). The DAP12 protein structure consists of a short extracellular domain, a transmembrane domain, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM) (2). DAP12 protein is expressed by hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, mast cells, basophils, eosinophils, neutrophils, and some γδ T cells and NKT cells (1). DAP12 exists as a homodimer that associates with a variety of receptors involved in pathogen detection, including the KIR family of NK cell receptors (2,3). Ligand binding by DAP12-associated receptors results in phosphorylation of tyrosine residues within the DAP12 ITAM by Src family kinases and leads to activation of Syk or Zap-70 and downstream signaling responses (2). Alternate Names DAP12; DNAX adaptor protein 12; DNAX-activation protein 12; KAR-associated protein; KARAP; killer activating receptor associated protein; Killer-activating receptor-associated protein; PLOSL; PLOSL1; polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; transmembrane immune signaling adaptor TYROBP; TYOBP; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG