CST, 4612S, CTMP Antibody

Polyclonal Antibody for studying CTMP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CTMP Antibody (CST #4612) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CTMP Antibody detects endogenous levels of total CTMP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human CTMP. Antibodies are purified by protein A and peptide affinity chromatography. Background Carboxyl-terminal modulator protein (CTMP) is a negative regulator of Akt and was identified as an Akt binding protein. Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase, activated by insulin and various growth and survival factors, functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4), and by phosphorylation within the carboxy-terminus at Ser473. CTMP binds to the regulatory domain of Akt at the carboxy terminus of the protein and inhibits phosphorylation on Thr308 and Ser473 (5). Alternate Names Acyl-CoA thioesterase THEM4; Acyl-coenzyme A thioesterase THEM4; C-terminal modulator protein; Carboxyl-terminal modulator protein; CTMP; MGC29636; THEM4; Thioesterase superfamily member 4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22, 26 SOURCE: Rabbit