CST, 46134SF, Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ACPP. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #46134) is ready to ship. Product Usage Information This product is the carrier free version of product #12861. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12861 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ACPP protein. This antibody is predicted to detect both the cellular and secreted isoforms of ACPP. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human full-length prostatic acid phosphatase. Background Prostatic Acid Phosphatase (ACPP or PAP) is a member of the histidine acid phosphatase family. It is a non-specific phosphatase that is capable of dephosphorylating tyrosine residues as well as phospholipids under mildly acidic conditions. ACPP has ecto-5'-nucleotidase activity in pain-sensing neurons where it converts AMP to adenosine, suppressing the pain response (1,2). ACPP occurs as two isoforms that are both heavily glycosylated. The secreted phosphatase (sPAP) is found predominantly in the prostate and seminal plasma, while the cellular isoform (cPAP) is broadly expressed at very low levels and is associated with the plasma and lysosomal membranes (3-5). Cellular PAP has been shown to dephosphorylate ErbB2 at various tyrosine residues effectively terminating signaling (6). Furthermore, the physical interaction between cPAP and ErbB2 appears to regulate androgen sensitivity in prostate cancer cells. Loss of cPAP in androgen-sensitive prostate cancer cells results in the development of a castration-resistant phenotype suggesting that ACPP plays a significant role in prostate cancer cell growth (7). ACPP is expressed in metastatic cells arising from prostate cancer - especially in prostate-derived bone metastasis - suggesting that it may be a relevant diagnostic indicator of prostate cancer re-emergence in bone (8). Alternate Names 5'-NT; 5'-nucleotidase; acid phosphatase 3; acid phosphatase, prostate; ACP-3; ACP3; ACPP; Ecto-5'-nucleotidase; PAP; PAPf39; PPAP; Prostatic acid phosphatase; prostatic acid phosphotase; Thiamine monophosphatase; TM-PAP; TMPase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG