CST, 46141T, MPC2 (D4I7G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MPC2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MPC2 (D4I7G) Rabbit Monoclonal Antibody (CST #46141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MPC2 (D4I7G) Rabbit Monoclonal Antibody recognizes endogenous levels of total MPC2 protein. This antibody does not cross-react with MPC1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn33 of human MPC2 protein. Background The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the citric acid cycle generate energy through oxidative phosphorylation under aerobic conditions (1,2). Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2), form a 150 kDa complex and are essential proteins in the facilitated transport of pyruvate into mitochondria (1,2). Mutations in the corresponding gene are associated with deficient pyruvate transport and may result in lactic acidosis, developmental delay, and premature death (2,3). Altered MPC1/MPC2 expression or activity may result in significant metabolic disorders and contribute to the increase in aerobic glycolysis in cancer cells (a.k.a., the Warburg effect) (4). Alternate Names BR44; Brain protein 44; BRP44; DKFZp564B167; MGC125752; MGC125753; Mitochondrial pyruvate carrier 2; MPC2; SLC54A2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG