CST, 46147T, TRIM33 (F9P6E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM33 mouse. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM33 (F9P6E) Rabbit Monoclonal Antibody (CST #46147) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TRIM33 (F9P6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM33 protein. This antibody is not recommended for use with mouse samples using flow cytometry. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TRIM33 protein. Background The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain (1). Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability (2). TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex (3); it functions as a corepressor for Krüppel-associated box (KRAB) zinc-finger transcription factors (4). Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 (5). TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ (6). Alternate Names 8030451N04Rik; AI413936; DKFZp586K1123; E3 ubiquitin-protein ligase TRIM33; Ect; Ecto; ectode; Ectodermin homolog; Kiaa1113; mKIAA1113; RING-type E3 ubiquitin transferase TRIM33; Tif1; TIF1-gamma; Tif1g; Transcription intermediary factor 1-gamma; TRI33; Trim33; tripartite motif protein 33; tripartite motif-containing 33; Tripartite motif-containing protein 33 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG