CST, 4618S, MAML2 Antibody

Polyclonal Antibody for studying MAML2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MAML2 Antibody (CST #4618) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAML2 Antibody detects endogenous levels of total MAML2 protein. This antibody does not cross-react with MAML1 and MAML3. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp800 of human MAML2. Antibodies are purified by protein A and peptide affinity chromatography. Background Mastermind-like (MAML) family of proteins are homologs of Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7). Alternate Names DKFZp686N0150; KIAA1819; Mam-2; MAM-3; MAM2; MAM3; MAML2; mastermind like transcriptional coactivator 2; mastermind-like 2; mastermind-like 2 (Drosophila); Mastermind-like protein 2; MGC176701; MLL-MAML2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit