CST, 46307T, IL-2R beta (D4X3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL2RB. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-2R beta (D4X3H) Rabbit Monoclonal Antibody (CST #46307) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100 μg/ml, 50% glycerol and less than 0.02% sodium azide. Store at -20ºC. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IL-2R beta (D4X3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-2Rβ protein. This antibody cross-reacts with proteins of unknown identity at 38-42 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu428 of human IL-2Rβ protein. Background Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). IL-2Rβ, also known as CD122, is a type I transmembrane protein that is expressed by natural killer cells and certain subsets of T cells in the periphery (6). IL-2Rβ surface expression is upregulated following antigen-induced activation. A heteromeric complex composed of IL-2Rβ and γc forms the intermediate affinity IL-2 receptor whereas a complex comprised of all three chains forms the high affinity receptor. Signaling through IL-2Rβ is mediated by Jak1 (7), which phosphorylates numerous tyrosine residues in the cytoplasmic tail to promote the recruitment of the adaptor Shc to IL-2Rβ and enhance IL-2 receptor signaling (8, 9). Alternate Names CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IL15RB; IL2RB; IMD63; interleukin 15 receptor, beta; interleukin 2 receptor subunit beta; interleukin 2 receptor, beta; Interleukin-15 receptor subunit beta; Interleukin-2 receptor subunit beta; p70-75; p75 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 79 Source/Isotype: Rabbit IgG