CST, 4632S, GADD45 alpha (D17E8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GADD45A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GADD45 alpha (D17E8) Rabbit Monoclonal Antibody (CST #4632) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GADD45 alpha (D17E8) Rabbit Monoclonal Antibody detects endogenous levels of total GADD45 alpha protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asn129 of human GADD45 α. Background GADD45 α, β and γ are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4). Alternate Names DDIT-1; DDIT1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; GA45A; GADD45; GADD45A; growth arrest and DNA damage inducible alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible, alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG