CST, 4636S, MAD2L1 (D8A7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAD2L1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MAD2L1 (D8A7) Rabbit Monoclonal Antibody (CST #4636) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MAD2L1 (D8A7) Rabbit Monoclonal Antibody detects endogenous levels of total MAD2L1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MAD2L1 protein. Background Correct segregation of sister chromatids prior to the onset of cell division is essential to the maintenance of genetic integrity and the avoidance of aneuploidy and chromosomal instability, characteristics of many cancer cells. The mitotic checkpoint, also known as the spindle assembly checkpoint, monitors accurate attachment of kinetochores to the spindle, inhibits mitosis and delays the onset of anaphase until all chromosomes are aligned at the metaphase plate (1). MAD2L1 is an essential participant in the mitotic checkpoint (2). It exists in two conformations, including the open and inactive O-MAD2 form and the closed, active C-MAD2 form. Prior to mitosis, MAD2L1 is localized to the cytosol and exists largely in the closed, inactive form. During the mitotic checkpoint, MAD2L1 switches to the open, active conformation (3). Together with other checkpoint proteins, MAD2L1 binds to and deactivates Cdc20, thereby inhibiting the anaphase promoting complex (4). When the kinetochores are correctly attached to the spindle, MAD2L1 releases Cdc20, which allows activation of the anaphase promoting complex and subsequent degradation of key mitotic substrates and the initiation of metaphase-anaphase transition (5). Alternate Names HsMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2-like protein 1; MAD2L1; MD2L1; mitotic arrest deficient 2 like 1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG