CST, 46386S, NHE1 (E2S8S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NHE1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NHE1 (E2S8S) Rabbit Monoclonal Antibody (CST #46386) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NHE1 (E2S8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total NHE1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NHE1 protein. Background The sodium/hydrogen exchanger (NHE) family of proteins are membrane-bound antiporters that extrude extracellular Na in exchange for intracellular H (1). By exchanging protons for sodium ions, NHE plays an important role in diverse physiological processes, including cell cycle, size, and proliferation, vesicle tracking and biogenesis, and ion homeostasis. The mammalian genome contains 13 NHE family members encoded by the gene family. NHE1, encoded by the gene, has a complex structure consisting of 13 transmembrane regions (2,3). Dysfunction of NHE1 is implicated in tumor progression and is genetically linked to several neurological disorders, including Lichtenstein-Knorr syndrome (4,5). Alternate Names APNH; APNH1; FLJ42224; LIKNS; Na-Li countertransporter; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na/H+ exchanger; Na+/H+ antiporter, amiloride-sensitive; NHE-1; NHE1; PPP1R143; protein phosphatase 1, regulatory subunit 143; SL9A1; SLC9A1; Sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9 member A1; solute carrier family 9, isoform A1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80-110 Source/Isotype: Rabbit IgG