CST, 46419S, Tox4 (E9U3L) Rabbit Monoclonal Antibody (ChIP Formulated)

Monoclonal Antibody for studying Tox4. Validated for Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Tox4 (E9U3L) Rabbit Monoclonal Antibody (ChIP Formulated) (CST #46419) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Chromatin IP Specificity / Sensitivity Tox4 (E9U3L) Rabbit Monoclonal Antibody (ChIP Formulated) recognizes endogenous levels of total Tox4 protein. This antibody does not cross-react with human Tox, Tox2, or Tox3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val583 of human Tox4 protein. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox4 is a regulatory factor of PP1 phosphatases and plays important roles in transcription, which include restricting pause release and early productive elongation, facilitating transcriptional reinitiation, and promoting late productive elongation (3). Tox4 has been shown to modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (4). Tox4 is a regulator of hepatic glucose production in an insulin receptor and FoxO1-independent manner, and its expression is increased in the liver in diabetic patients with nonalcoholic fatty liver disease (NAFLD) (5). Alternate Names C14orf92; Epidermal Langerhans cell protein LCP1; KIAA0737; LCP1; MIG7; migration-inducing protein 7; TOX high mobility group box family member 4; TOX4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG