CST, 46454S, MTF2 (F2X1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTF2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, MTF2 (F2X1A) Rabbit Monoclonal Antibody (CST #46454) is ready to ship. Product Usage Information For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MTF2 (F2X1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly521 of human MTF2 protein. Background Metal regulatory transcription factor 2 (MTF2), also referred to as polycomb-like 2 (PCL2), is a catalytically inactive member of the PCL protein family (1). MTF2 binds to the metal response element of the mouse metallothionein promoter (2,3) and has also been shown to regulate the PRC2 activity at specific developmental genes in embryonic stem cells (4,5). It was also demonstrated that MTF2 promotes the proliferation and invasion of hepatocellular carcinoma cells (6) and that the MTF2-MDM2 axis can be targeted to sensitize refractory acute myeloid leukemia (AML) to chemotherapy (7). Alternate Names dJ976O13.2; hPCl2; M96; Metal regulatory transcription factor 2; metal response element binding transcription factor 2; metal response element-binding transcription factor 2; Metal-response element DNA-binding protein M96; Metal-response element-binding transcription factor 2; MTF2; PCL2; polycomb-like 2; Polycomb-like protein 2; putative DNA binding protein; RP5-976O13.1; TDRD19A; tudor domain containing 19A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50, 55, 70 Source/Isotype: Rabbit IgG