CST, 4648S, DHX29 (G63) Antibody

Polyclonal Antibody for studying DHX29. Validated for Western Blotting. Highly specific and rigorously validated in-house, DHX29 (G63) Antibody (CST #4648) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DHX29 (G63) Antibody detects endogenous levels of total DHX29 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Gly63 of human DHX29 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2). Alternate Names ATP-dependent RNA helicase DHX29; DDX29; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29; DEAH (Asp-Glu-Ala-His) box polypeptide 29; DEAH box protein 29; DEAH-box helicase 29; DExH-box helicase 29; DHX29; FLJ21492; Nucleic acid helicase DDXx Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 155 SOURCE: Rabbit