CST, 4655S, TESK1 (D49D4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TESK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TESK1 (D49D4) Rabbit Monoclonal Antibody (CST #4655) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TESK1 (D49D4) Rabbit Monoclonal Antibody detects endogenous levels of total TESK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy terminal residues of human TESK1 protein. Background Testis-specific kinase 1 (TESK1) is a LIMK-related protein kinase originally identified to be expressed highly in testes and subsequently shown to be expressed in a wide variety of tissues and cell types (1-4). TESK1 phosphorylates the actin severing protein cofilin at Ser3, inactivating cofilin and thus regulating the organization of the actin cytoskeleton (2). Integrin signaling activates TESK1 activity and leads to stress fiber formation and cell spreading (2,5,6). TESK1 is involved in regulation of ERK signaling through its interaction with Spry2 (7) and regulation of cell spreading through its interaction with the focal adhesion protein actopaxin/α-parvin (5). Alternate Names Dual specificity testis-specific protein kinase 1; TESK1; Testicular protein kinase 1; testis associated actin remodelling kinase 1; testis-specific kinase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG