CST, 4656T, Cyclin A2 (BF683) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Cyclin A2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin A2 (BF683) Mouse Monoclonal Antibody (CST #4656) is ready to ship. Product Usage Information Western Blotting: 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cyclin A2 (BF683) Mouse Monoclonal Antibody detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein. Background While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition, causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for the completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5). Alternate Names CCN1; CCNA; CCNA2; Cyclin A; cyclin A2; Cyclin-A; Cyclin-A2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Mouse IgE