CST, 46592S, CD62L/L-Selectin (MEL-14) Rat Monoclonal Antibody (APC Conjugate)

Monoclonal Antibody for studying L-Selectin mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD62L/L-Selectin (MEL-14) Rat Monoclonal Antibody (APC Conjugate) (CST #46592) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.06μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:300 Flow Cytometry (Live): 1:300 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) recognizes endogenous levels of total mouse CD62L protein. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6). Alternate Names AI528707; CD62; CD62 antigen-like family member L; CD62L; L-sel; L-selectin; LAM-1; LECAM; LECAM-1; LECAM1; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; Ly-2; Ly-22; Ly-m2; Ly-m22; Ly22; Lya; Lyam; Lyam-1; Lyam1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; selectin, lymphocyte; Sell Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2a kappa