CST, 4664S, ABIN-1 Antibody

Polyclonal Antibody for studying ABIN1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ABIN-1 Antibody (CST #4664) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ABIN-1 Antibody detects endogenous levels of total ABIN-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ABIN-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-κB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5). ABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7). Overexpression of ABIN-1 inhibits NF-κB activation by a number of stimuli including TNF, IL-1, and LPS. It is widely expressed and can be induced by NF-κB, providing a negative feedback loop of NF-κB signaling. In addition to binding to A20, ABIN-1 can function on other key components of NF-κB signaling including IKKγ/NEMO (8) and NF-κB family members p100 and p105 (9), and can also inhibit ERK signaling (10). Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11). ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis. Alternate Names A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; hVAN; KIAA0113; NAF1; Nef-associated factor 1; Nef-associated factor 1 SNP; Nip40-1; TNFAIP3 interacting protein 1; TNFAIP3-interacting protein 1; TNIP1; VAN; Virion-associated nuclear shuttling protein; virion-associated nuclear-shuttling protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80-85 SOURCE: Rabbit