CST, 46653T, FBXL11 (F6R4Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FBXL11. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, FBXL11 (F6R4Y) Rabbit Monoclonal Antibody (CST #46653) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity FBXL11 (F6R4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total FBXL11 protein. This antibody detects a 180 kDa band of unknown origin in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val530 of human FBXL11 protein. Background F-box and leucine-rich repeat protein 11 (FBXL11), also known as lysine-specific demethylase 2A (KDM2A) and JmjC domain-containing histone demethylation protein 1A (JHDM1A), is a lysine-specific histone demethylase protein that demethylates Lys4 and Lys36 of histone H3 (1,2). FBXL11 maintains heterochromatin and promotes epigenetic silencing by associating with centromeres and recruiting HP1 (3). Furthermore, FBXL11 is recruited to non-methylated CpG islands by its zinc finger CxxC domain (3). FBXL11 can also control ribosome biogenesis by preventing the transcription of rRNA genes (4). During DNA damage, FBXL11 is phosphorylated by ATM, which reduces its ability to bind to chromatin, allowing H3K36 dimethylation to accumulate and recruit the MRE11 complex (5). Additionally, there is a short isoform of FBXL11 that lacks a JmjC domain and histone demethylation activity. The short isoform has been implicated in breast cancer progression and binds in distinct complexes to HP1alpha (6,7). Alternate Names [Histone-H3]-lysine-36 demethylase 1A; CXXC-type zinc finger protein 8; CXXC8; DKFZp434M1735; F-box and leucine-rich repeat protein 11; F-box protein FBL11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; FBL11; FBL7; FBXL11; FLJ00115; FLJ46431; JHDM1A; JmjC domain-containing histone demethylation protein 1A; jumonji C domain-containing histone demethylase 1A; KDM2A; KIAA1004; LILINA; lysine (K)-specific demethylase 2A; lysine demethylase 2A; Lysine-specific demethylase 2A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 138 Source/Isotype: Rabbit IgG