CST, 4666S, WRN (8H3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying WRN. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, WRN (8H3) Mouse Monoclonal Antibody (CST #4666) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51201 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity WRN (8H3) Mouse Monoclonal Antibody detects endogenous levels of total WRN protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with full length human recombinant WRN protein. Background The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes, including double-strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members result in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). is a member of the RecQ family that has been identified as the gene underlying Werner's syndrome, an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner's syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5). Alternate Names Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DKFZp686C2056; DNA helicase, RecQ-like type 3; Exonuclease WRN; RecQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; Werner syndrome, RecQ helicase-like; WRN; WRN RecQ like helicase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Mouse IgG1